![]() ![]() Anti-Mullerian hormone is highly associated with superovulation response and oocyte and embryo production, and its use should improve animal selection to achieve improve efficiency of multiple-ovulation embryo transfer. Circulating AMH is stable over time in unstimulated animals but decreases in repetitively stimulated animals. A significant difference was observed between the high- and normal-AMH categories (12 ± 3.6 v. As OPU animals with low AMH were used only once, average oocyte/collection data was not available for this category. When separated into AMH groups, high-AMH flushed animals had significantly higher numbers of embryos than the normal- or low-AMH animals (9.3 ± 3.1 v. Comparison of the first AMH value to the average number of oocytes or embryos collected over the course of 3 collections/animal showed a positive correlation to the average number of oocytes/collection from individual OPU donors ( r 2 = 0.436) and a positive correlation to the average number of embryos/collection from individual donors ( r 2 = 0.176). Company Profile Verona, WI Competitors, Financials & Contacts - Dun & Bradstreet Find company research, competitor information, contact details & financial data for companyname of companycitystate. Flushed animals with high AMH levels had significantly higher numbers of embryos than those with low AMH (10.9 ± 7.9 v. The statistical analyses (ANOVA and data correlation) were performed using Statview 5 with P 400 pg mL –1), high-AMH OPU animals yielded significantly higher numbers of oocytes than the animals in the normal or low AMH groups (13.8 ± 9.2 v. The samples were stored at –20☌ until evaluation using the Minitube of America AMH-bovine specific immunoassay (AMH Fertility Assay™). (Minitube of America Inc., Verona, WI) and allow semen collec-tion by the gloved hand technique at approximately 7 mo of age. Blood samples were collected in serum tubes and spun within 2 h. Animals were followed over 3 consecutive cycles induced using a modified Ovsynch protocol with 4 days of FSH (Pluset H, Minitube of America, Verona, WI, USA). Forty-one superovulated heifers were evaluated at ovum pick-up (OPU) performed 20 h after cystorelin administration, and 125 others were evaluated at embryo recovery. The AMH levels of 15 unstimulated cows were followed at weekly intervals during their oestrous cycles and monthly for 4 months. ![]() The relations between serum anti-Mullerian hormone (AMH), oocyte numbers, and in vivo embryo production in Holstein heifers were evaluated. ![]()
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